ELEKTROFOREZA KAPILARNA PDF

Title (Croatian), Kapilarna elektroforeza u farmaciji. Author, Damić, Miranda Nigović, Biljana. Abstract (Croatian). Capillary electrophoresis is a new separation. Elektroforeza kapilarna (CE) to metoda analityczna o bogatej historii. Jest popularna w laboratoriach ze względu na swój ekologiczny charakter, niezawodność. Kupryszewski, J. Barciszewski, Capillary electrophoresis: a new tool for biomolecule separation (Elektroforeza kapilarna: nowe narzedzie analizy biomolekul).

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Disable autocomplete Translate upon paste Select term in search box. To improve the sensitivity, the path length can be increased, though this results in a loss of resolution.

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Kapilarna elektroforeza

The capillary tube itself can be expanded at wlektroforeza detection point, creating a “bubble cell” with a kapilaran path length or additional tubing can be added at the detection point. EUdict European dictionary is a collection of online dictionaries for the languages spoken mostly in Europe.

Main advantages over other chromatographic methods are short analysis time, high efficiency, small sample and solvent volumes, low costs, simplicity and ecological acceptability. Introduced in the s, the technique of capillary electrophoresis CE was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte. Adding a surfactant to the electrolyte can facilitate the separation of neutral compounds by micellar electrokinetic chromatography.

There are several ways to use this dictionary. Osnovne komponente kapilarne elektroforeze so vir visoke napetosti, dve elektrodi, dva rezervoarja s pufrom, kapilara, detekcijski sistem in sistem za elektrofroeza in obdelavo signala slika 1.

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Elektroosmozni tok je pomemben pri vrednostih pH nad 3. Delce z nabojem poganja proti elektrodi sila Eq. Postopek analize poteka tako, da kapilaro napolnimo s pufrom in vanjo injiciramo nekaj nanolitrov vzorca. Fundamentals of Analytical Chemistry, 8.

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In traditional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. Besedilo se sme prosto uporabljati v skladu z dovoljenjem Creative Commons Priznanje avtorstva-Deljenje pod enakimi pogoji 3.

Capillary electrophoresis CE can be used to separate ionic species by their charge and frictional forces. My name is Tomislav Kuzmic, I live in Croatia and this site is my personal project. However, there are several techniques that can help separate such analytes with a capillary electrophoresis system.

J Chromatogr A I am responsible for the concept, design, programming and development.

This set-up requires volatile buffer solutions, which will affect the range of separation modes that can be employed and the degree of resolution that can be achieved. Capillary electrophoresis has several modes of separation which allows analysis of different types of analytes. Za podrobnosti glej Pogoje uporabe.

kapilarna elektroforeza | EUdict | Croatian>English

Charged polymers such as DNA can be separated by filling the capillary with a gel matrix that retards longer strands more than shorter strands. The system’s main components are a sample vial, source and destination vials, a capillary, electrodes, a high-voltage power supply, a detector, and a data output and handling device.

Trans Faraday Soc Despites of all advantages and application possibilities, capillary electrophoresis is still not enough explored and used in routine analysis. Hitrost potovanja delca definiramo z elektroforezno mobilnostjo, ki predstavlja razmerje med hitrostjo potovanja?

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The resulting ions are then analyzed by the mass spectrometer.

The analytes separate as they migrate due to their electrophoretic mobility, as will be explained, and are detected near the outlet end of the capillary. Related techniques As discussed above, separations in a capillary electrophoresis system are typically dependent on the analytes having different electrophoretic mobilities. To introduce the sample, the capillary inlet is placed into a vial containing the sample and then returned to the source vial sample is introduced into the capillary via capillary action, pressure, or siphoning.

The data is then displayed as an electropherogram, which reports detector response as a function of time. E – Elektroforeza kapilarna ang.

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Elektroosmoza

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In order to obtain the identity of sample components, capillary electrophoresis can be directly coupled with mass spectrometers or Surface-Enhanced Raman Spectroscopy SERS.